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The monocytes (2x10 6 )/well were seeded in wells of six-well plates and cultured in a nutrient-rich culture medium (RPMI1640 medium supplemented with 10% calf serum, 2 mM mercaptoethanol, 1 mM glutamine, 100 unit/ml penicillin G, and 100 μg/ml streptomycin sulphate) and a DC growth stimulant mix of GM-CSF and IL-4