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Biotinylation Purified StrepII–Avi-tagged DCAF11 or DDX18 were biotinylated in vitro by incubation with final concentrations of 2.5 μM BirA enzyme (prepared in-house) and 0.2 mM biotin in 50 mM HEPES, pH 7.4, 200 mM NaCl, 10 mM MgCl 2 , 1 mM TCEP and 20 mM ATP

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[62] mTOR has emerged as an attractive target for the development of anticancer agents due to its crucial role in regulating cell growth, proliferation, metabolism, and immunity