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This figure was much closer to that observed with our original conditions (50 μM, 30′), with the remaining signal in the other two conditions (50 μM, 60′ and 250 μM, 30′) being rather higher

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Additionally, gentamicin has a glomerular effect that impairs filtration, causes mesangial contraction, and reduces the ultrafiltration coefficient and glomerular filtration rate [ 48 , 49 ]