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Immunohistochemical analysis and section staining Mice in the various treatment groups were anesthetized with pentobarbital sodium (Sigma-Aldrich, USA) and perfused with phosphate-buffered saline (PBS), followed by 4% paraformaldehyde in PBS

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A number of compounds were detected in the frass of larvae fed on a diet with 4MSOB ITC, including 4MSOB ITC, 4MSOB ITC-GSH, 4MSOB ITC-CysGly, 4MSOB ITC-Cys, and 4MSOB ITC-Cyclic-Cys (Fig