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While the exact cause of anhedonia can, at times, be uncertain (when there is no proper diagnosis), the inherent association between pleasure modulation, the brain’s reward system, and many neurobiological factors has led some researchers to further investigate whether anhedonia is a result of any of the following conditions:[3] Reduced activity of the brain regions Imbalances and deficits in neurotransmitters Imbalances in the immune system To best understand how these factors intersect, it is important to first be able to understand the brain’s reward system and how humans are biologically motivated by reward