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Cell culture and viability assay HEI-OC1 auditory cells were cultured under permissive conditions (33 °C, 10% CO 2 ) for 16 h in Dulbecco’s modified Eagle’s medium (Hyclone, Logan, UT, USA) containing 10% fetal bovine serum (Hyclone, Logan, UT, USA) and 50 units/mL IFN-γ (PeproTech, EC, London, UK) without antibiotics

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doi: 10.2527/jas.2005-311